Coding

Part:BBa_K1039004:Design

Designed by: Urooj Kishor   Group: iGEM13_Waterloo   (2013-09-10)


Bxb1 Invertible Promoter Switch (Promoter J23119) (RL State)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 337
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 539
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The transcriptional terminator J61048 was included to ensure transcription only proceeds in the expected direction. Spacer regions were added adopted from Bonnet lab.


Source

The sequence was designed by Bonnet lab at the Department of Bioengineering, Stanford University, and was further modified by Waterloo team.

References